Journal: The Journal of Neuroscience
Article Title: Matrix Metalloproteinase-9 Undergoes Expression and Activation during Dendritic Remodeling in Adult Hippocampus
doi: 10.1523/JNEUROSCI.22-03-00920.2002
Figure Lengend Snippet: MMP-9 is localized in neuronal processes of cultured hippocampal neurons. Co-immunostaining of dissociated hippocampal neurons in culture with anti-MMP-9 antibody Ab.1 and anti-TUJ1 antibody is shown. Although dissociated neurons did not show clear neuritic expression of the enzyme at low magnification (top images; scale bar, 500 μm), when the cells were analyzed at high magnification, MMP-9 protein was localized clearly in TUJ1-positive neuronal processes (bottom images; scale bar, 50 μm).
Article Snippet: Cultures were fixed with 4% paraformaldehyde in PBS, washed, and co-immunostained as described above with anti-MMP-9 Ab.1 and a monoclonal antibody against neuron-specific type III β-tubulin; (TUJ1; 1:500; Covance).
Techniques: Cell Culture, Immunostaining, Expressing